Use of a Fiber Optic Spectrometer to Assess the Minimum Number of Malignant Cells by Diffuse Reflectance Spectra in the Near Infrared Region
https://doi.org/10.17586/0021-3454-2023-66-2-148-154
Abstract
The aim of the study was to develop a technique for assessing the minimum number of malignant cells recognizable by their diffuse reflectance spectra obtained in the near infrared region (NIR) using compact spectrometric equipment. In the course of the study, a culture of ovarian cancer cells was prepared for subcutaneous injection into la- boratory mice; solutions with various concentrations of such cells were prepared to create a calibration scale. Measure- ment of NIR spectra was carried out on live laboratory mice (at pre-marked points) under anesthesia before puncture and after the introduction of cell culture. Spectral characteristics that are sensitive to changes in the number of cells at the measurement point (peak intensity, baseline shift) have been identified, and the minimum reliably recognizable number of malignant tumor cells has been estimated from the graph of the dependence of the absorption value at a wavelength of 1200 nm on the logarithm of the number of cells in solution.
Supporting agencies: The study was supported by a grant from the President of the Russian Federation No. MK2192.2021.4
About the Authors
E. S. Boichenko
ITMO University
Russian Federation
Russian Federation
Ekaterina S. Boichenko — Research Engineer
St. Petersburg
A. V. Panchenko
Research Institute of Medical Primatology
Russian Federation
Russian Federation
Andrey V. Panchenko — Dr. Sci.; Chief Researcher
Sochi
A. A. Surkova
Samara State Technical University
Russian Federation
Russian Federation
Anastasia A. Surkova — PhD; Researcher
Samara
D. O. Kirsanov
St. Petersburg State University
Russian Federation
Russian Federation
Dmitry O. Kirsanov — Dr. Sci., Professor
St. Petersburg
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Review
For citations:
Boichenko E.S., Panchenko A.V., Surkova A.A., Kirsanov D.O. Use of a Fiber Optic Spectrometer to Assess the Minimum Number of Malignant Cells by Diffuse Reflectance Spectra in the Near Infrared Region. Journal of Instrument Engineering. 2023;66(2):148-154. (In Russ.) https://doi.org/10.17586/0021-3454-2023-66-2-148-154
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